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Pumilacidin-related mass peaks were detected in acid precipitation extracts through MALDI-TOF analysis. Most strains showed several common BGCs, including bacteriocin, polyketide synthase (PKS), and non-ribosomal peptide synthetase (NRPS), related to pumilacidin, bacillibactin, bacilysin, and other antimicrobial compounds. These isolates were identified as Bacillus safensis (3), Bacillus pumilus (1), and Bacillus megaterium (1), using the genomic metrics ANI and dDDH. CodonCode Aligner introduced several features and improvements like building Neighbor-Joining trees, zooming in the contig view, shortening reference sequences after alignments, masking contig bases in low coverage regions, the percentange consensus as additional consensus method, and much more. All antagonistic isolates belonged to the Bacillus genus, and had their genomes sequenced for the search of biosynthetic gene clusters (BGC) related to antimicrobial metabolites.
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sclerotiorum by the dual culture assay and four of them also inhibited fungal growth by volatile organic compounds production. Five isolates presented antifungal activity against S. Several isolates produced auxin, siderophores, hydrolytic enzymes, fixed nitrogen and solubilized phosphate. pollen (BCP) the reproductive cell divides to give tricellular pollen (TCP).
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BLAST, basic local alignment search tool coa, coatlique mutant dCHIP, DNA.
#Codoncode aligner blast tcp code
Thirty isolates belonging to Bacillus, Paenibacillus, Lysinibacillus, and Microbacterium genera were obtained. Sequences were processed and aligned with Codon Code Aligner V5.0.2. LIKE 3 ATX3 1.47-fold) and AT5G50915 (encoding a TCP transcription. Bacteria from canola roots were isolated and screened for different plant growth promotion (PGP) traits and biocontrol of Sclerotinia sclerotiorum. Endophytic bacteria show important abilities in promoting plant growth and suppressing phytopathogens, being largely explored in agriculture as biofertilizers or biocontrol agents.